| Autor: |
Timofeev, Sergey A., Shukhalova, Anastasia G., Senderskiy, Igor V., Mitina, Galina V., Gannibal, Philipp B., Dolgikh, Viacheslav V. |
| Zdroj: |
BioControl; Feb2024, Vol. 69 Issue 1, p65-75, 11p |
| Abstrakt: |
The utilization of diverse entomopathogens like bacteria, viruses, or fungi holds promise in the biological control of insect pests. One approach to enhance their effectiveness involves boosting the virulence of these pathogens through genetic modification. This is frequently accomplished by integrating a sequence into the pathogen's genome that encodes an effector molecule capable of negatively impacting the host insect. In this study, we examined two neurotoxins isolated from the venom of the ectoparasitoid wasp Habrobracon hebetor (Hymenoptera: Braconidae), previously described in a series of expired patents. These protein toxins, with molecular weights of 30 kDa (HhT30) and 16 kDa (HhT16), were both expressed in bacteria to produce antibodies. They were also expressed in Sf9 insect cell culture using a baculovirus system as a model for generating viral preparations based on them, as well as in entomopathogenic fungi Beauveria bassiana and Akanthomyces muscarius (formerly, Lecanicillium muscarium) (Hypocreales: Cordycipitaceae) with the intention of augmenting their virulence. This research has demonstrated that while these molecules naturally exhibit a toxic impact on the nervous system of insects attacked by the parasitoid through hemolymph, they seem to exert a substantial toxic effect on various organisms at the cellular level being heterologously expressed. Among all the methods tested, only the use of HhT30 for genetic modification of entomopathogenic baculoviruses appears to be promising. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
Full text from SpringerLink