A facile method to determine the molar mass of soft nanoparticles.

Autor:	Schmidt, Thomas, Hohenschutz, Max, Becker, Moritz, Grabowski, Frédéric, Jupke, Andreas, Pich, Andrij, Wöll, Dominik
Předmět:	MOLAR mass STAINS & staining (Microscopy) NANOPARTICLES OPTICAL microscopes RESEARCH personnel
Zdroj:	Colloid & Polymer Science; Mar2024, Vol. 302 Issue 3, p345-354, 10p
Abstrakt:	The determination of the molar mass of soft nanoparticles is essential to estimate their (molar) concentration in dispersions. Measuring this quantity by conventional methods, however, often proves challenging. We describe a facile approach to determine the molar mass of (soft) nanoparticles via counting their number per volume using a widefield fluorescence microscope. The method is exemplified on a microgel dispersion as a model system, while it is applicable to other types of stainable nanoparticles. For this, covalent labeling or modification of the nanoparticles is not required. The dispersion is simply mixed with a Nile Red solution in a defined ratio and measured in an optical fluorescence microscope accessible to most researchers in the field. [ABSTRACT FROM AUTHOR]
Databáze:	Complementary Index
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