| Autor: |
Zagoskina, N. V., Zubova, M. Y., Aksenova, M. A., Nechaeva, T. L., Zaitsev, G. P. |
| Předmět: |
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| Zdroj: |
Biology Bulletin; 2023 Suppl 3, Vol. 50, pS373-S381, 9p |
| Abstrakt: |
The effect of different intensity of light exposure (5000 and 10 000 lux) on the growth of two strains (1 and 2) of the tea plant (Camellia sinensis L.) callus cultures and the accumulation of flavanols in them, including oligomeric forms—proanthocyanidins. The composition and content of monomeric flavanols—(+)-catechin and (–)-epicatechin in methanol extracts of cultures were studied by high-performance liquid chromatography (HPLC). Data on the morphology of calluses are presented and the greening of the strain 1 is noted, especially at high light intensity (10 000 lux). Its high ability to accumulate flavanols was established, which was 12–35 times higher than that of the strain 2. These differences depended on the age of the cultures and the intensity of light exposure. By HPLC method, monomeric flavanols—(+)-catechin and (–)-epicatechin were identified in methanol extracts of the callus strain 1, the amount of which increased at the end of the passage. Calluses of the low-producing strain 2 are characterized by the formation of only (–)-epicatechin, the amount of which did not change during their growth and did not depend on the intensity of light exposure. All this indicates significant differences in the accumulation of flavanols in the two strains of tea callus cultures and their response to light of different intensities. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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