Autor: |
GUO Yue, LU Xin-yu, WANG Ting, LI Wei-long, QI Yu-rong, LYU Xin-lei, PENG Qi, GAO Jian-qin, ZHANG Jie-fu, HU Mao-long |
Předmět: |
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Zdroj: |
Chinese Journal of Oil Crop Sciences; 2023, Vol. 45 Issue 6, p1206-1216, 11p |
Abstrakt: |
The service life of herbicide-resistant varieties can be prolonged by identification of intercrossing-resistance of existing herbicide-resistant rapeseed and the screening of application of other safe herbicide in dosage forms. In this study7 a new germplasm PN19 and transgenic plants of Arabidopsis and tobacco resistant to SU herbicides were used as materials. Five classes of ALS inhibitory herbicides with five concentration gradients were sprayed at 3-5 leaf stage of rapeseed. The intercrossing-resistance of PN19 to five herbicides was determined by resistance identification. Results showed that plant intercrossing-resistance against SU and SCT class was the strongest' followed by IMI and TP class7 whereas that against PB class was the lowest. Evaluation of herbicide intercrossing-resistance in transgenic plants and analysis of in vitro activity of tobacco ALS enzymes revealed this resistance function to various herbicides was resulted from the decreased sensitivity of target ALS enzymes to herbicides. In addition' the expression of PN19 herbicide-resistance gene after treatment with five herbicides was higher than that of wild type. The study provides an opportunity by mixing use of ALS herbicides to integratedly control weeds in the field. [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
Externí odkaz: |
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