| Autor: |
PENG Qi, ZHOU Xiao-ying, GAO Jian-qin, ZHANG Wei, SUN Cheng-ming, HU Mao-long, PU Hui-ming, GUO Yue, FU San-xiong, WANG Xiao-dong, ZHANG Jie-fu |
| Předmět: |
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| Zdroj: |
Chinese Journal of Oil Crop Sciences; 2023, Vol. 45 Issue 6, p1119-1127, 9p |
| Abstrakt: |
During the interaction between Brassica napus and Sclerotinia sclerotiorum, the expression of JA (jasmonic acid) synthesis-related genes increases, while JA signal transduction is inhibited. It is speculated that the effector proteins secreted by S. sclerotiorum may directly or indirectly interact with JAR1 protein, reducing its activity and thus inhibiting the defense system of B. napus dependent on JA pathway. The full-length sequence of the differentially expressed gene BnJarl was obtained by transcriptome sequencing in the early stage of this research. In order to further reveal the mechanism of BnJarl gene involved in the interaction between B. napus and S. sclerotiorum, this study analyzed and predicted the bioinformatics of BnJAR1 protein, constructed a yeast expression gene cDNA library of B. napus-S. sclerotiorum interaction, and used BnJar1 as bait protein to screen the library by yeast two-hybrid. Through the study, 5 interacting proteins from B. napus were obtained: BnAOC2, BnCOP9, BnDcoH, BnNIT2, and BnTSJT1; and 2 interacting proteins from S. sclerotiorum: SsMFS and SsRho3. The relevant results contributed to our research on the interaction between B. napus and S. sclerotiorum, exploration of pathogenic or resistance-related genes, and on further elucidation of their mechanisms. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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