Per-cell histone acetylation is associated with terminal differentiation in human T cells.

Autor: Yang, Cheng, Li, You, Hu, Yaqiu, Li, Qian, Lan, Yinghua, Li, Yongguo
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Zdroj: Clinical Epigenetics; 2/6/2024, Vol. 16 Issue 1, p1-10, 10p
Abstrakt: Background: Epigenetic remodeling at effector gene loci has been reported to be critical in regulating T cell differentiation and function. However, efforts to investigate underlying epigenetic mechanisms that control T cell behaviors have been largely hindered by very limited experimental tools, especially in humans. Results: In this study, we employed a flow cytometric assay to analyze histone acetylation at single-cell level in human T cells. The data showed that histone acetylation was increased during T cell activation. Among T cell subsets, terminally differentiated effector memory T (TEMRA) cells robustly producing effector cytokines were hyper-acetylated. Conversely, these TEMRA cells had lower expression levels of TCF-1, a key transcription factor for maintaining stem cell features. Pharmaceutical inhibition of histone acetylation using a small molecule C646 restrained the production of effector molecules, but retained stem cell-like properties in T cells after expansion. Conclusions: Per-cell histone acetylation is associated with terminal differentiation and poor stemness in human T cells. These observations suggest a new approach to enhance the stem cell-like properties of T cells and improve the efficacy of immunotherapy. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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