| Abstrakt: |
Red chilli (Capsicum annum L.) is a herbaceous plant with a spicy fruit taste caused by the content of capsaicin. One of the inhibiting factors for chilli production is a curly yellow disease caused by Pepper Yellow Leafcurl Indonesia Virus (PYLCIV) (Geminiviridae: Begomovirus). Symptoms of this disease are characterized by yellowing and curling of the leaves, changes in fruit size, stunted plant growth, reduced number of fruits produced, and reduced fruit size, resulting in loss of production of up to 80.82%. The whitefly (Bemisia tabaci Genn.), an insect vector, is well known for transmitting the curly yellow virus either in the nursery or in the field after transplanting. The presence of the PYLCIV virus has not been wholly detected in all chilli-growing centers in Indonesia. The aim of the present study is to determine the presence of the PYLCIV virus in chilli plants in Makassar as well as the insect vector, B. tabaci. A molecular method with PCR (Polymerase Chain Reaction) was performed for the detection and identification of the virus, with three primers: PYLCIV DNA A Primer, PYLCIV DNA B Primer, and General Primer SPG2. The results of PCR analysis showed that nine samples confirmed positive PYLCIV infection with bands measuring 693 bp DNA A, and 385 bp for DNA B. Phylogenetic analysis showed that Makassar isolate's were closely related to PYLCIV isolates of the Bali area. Therefore, it can be concluded that PYLCIV is existing in Makassar, South Sulawesi. [ABSTRACT FROM AUTHOR] |
