| Autor: |
Aksani, Dila, Natasia, Rena, Kusumati, Dinihari Indah, Tentrem, Titi, Susilowati, Dwi Ningsih, Harsonowati, Wiwiek, Yuniarti, Erny |
| Předmět: |
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| Zdroj: |
AIP Conference Proceedings; 2024, Vol. 2957 Issue 1, p1-8, 8p |
| Abstrakt: |
This research aimed to characterize the enzymatic activity potency of 10 isolates of Streptomyces, to test their antifungal activity against C. gloeosporioides, a red chili plant pathogen, and to test the ability of selected isolate in inhibiting C. gloeosporioides colonization on red chili seed germination. The enzyme activities tested were amylase, cellulase, protease, and chitinase. The antifungal activity test of the isolate against C. gloeosporioides used the dual culture method. The ability of selected isolates to inhibit pathogen colonization on seed germination was tested in a growth chamber experiment. The results showed that 8 isolates indicated amylase activity, while 4 isolates indicated cellulase and protease activity and 2 isolates (LB and ST20) indicated positive chitinase activity. Identified Streptomyces (S. prunicolor ST20 and S. andamanensis LB) were able to inhibit the fungus growth through non-volatile metabolites (7.9 and 50.2%) which was higher than through volatile metabolites (12.6 and 19%). Inhibition test of the pathogen colonization on the seed germination at 2 days after seed germination, all of the seed germination medium surfaces of control treatment were colonized by the fungus, while those on the treatments of Streptomyces inoculation had not been colonized. However, at 6 days after seed germination, the treated medium surface was colonized by the fungus at a lower intensity than the control treatment, whereas around the roots of ST20 inoculation had lower fungus spores than those of LB inoculation. In the future, we need to examine the fungus control by Streptomyces spp on seedling development and plant growth. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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