| Autor: |
Basuki, Sesanti, Cholid, Mohammad |
| Předmět: |
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| Zdroj: |
AIP Conference Proceedings; 2024, Vol. 2957 Issue 1, p1-11, 11p |
| Abstrakt: |
Nicotine is the main alkaloid compound produced by cultivated tobacco plants. Encoding genes involved in the biosynthetic pathway of nicotine have been studied. Single nucleotide polymorphisms (SNPs) are a class of mutations caused by substitution or insertion-deletion, and the most common and simplest type of genetic variation in organisms including plants. This study aims to identify and analyze the diversity of SNPs in the nicotine-related gene sequences from three different types of tobacco. The analysis resulted in 18 putative SNPs (local), 38 putative SNPs (flue-cured), and 15 putative SNPs (aromatic/semi-aromatic) with an average SNP density of one SNP in every 43 bases (local tobacco), one SNP for every 20 bases (flue-cured), and one SNP for every 51 bases (aromatic/semi-aromatic). Base substitutions are more common in transitions (70%) than that in transversion (30%). The nucleotide diversity of the gene fragments was ranging from 0.010 to 0.019. Each type of tobacco analyzed yielded six combinations of haplotypes with a frequency of 16.7% for local and flue-cured, and three combinations of haplotypes with a frequency of 33.3% for aromatic/semi-aromatic tobacco. Three indel types have also been identified in the gene fragment with approximately one indel every 18 bases. The natural indel and SNP variation associated with nicotine content serves as useful genetic information for breeding purposes. Moreover, the high allele variation caused by the single base substitution in the gene fragments could be used to develop an assisted selection tool based on SNPs markers that are associated with nicotine content. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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