| Autor: |
Nugroho, Kristianto, Terryana, Rerenstradika Tizar, Aswani, Nazly, Yusuf, Syah, M. Jawal Anwarudin, Prabawati, Sulusi, Yufdy, M. Prama, Roostika, Ika, Supriati, Yati, Lestari, Puji |
| Předmět: |
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| Zdroj: |
AIP Conference Proceedings; 2024, Vol. 2957 Issue 1, p1-8, 8p |
| Abstrakt: |
Garlic (Allium sativum L.) is one of the horticultural crops that is widely used as a seasoning in cuisine around the world. This study aimed to analyze the genetic diversity of Indonesia's local garlic accessions based on nucleotide variations in LECASAI and AsNF-YB3 gene fragments. The DNA from eleven Indonesian garlic local accessions were amplified using two pairs of specific gene markers designed from LECASAI (JX561228.1) and AsNF-YB3 (JX310756.1) gene sequences. The sequence alignment showed that a total of 14 nucleotide variations could be identified in this study as SNPs. As many as 12 SNPs were observed in the LECASAI gene fragment, while as many as two SNPs were observed in the AsNF-YB3 gene fragment. From eleven local garlic accessions used in this study, the highest variations were found in Eban NTT and Maja accessions with a percentage of variation of 33.33%, followed by Lumbu Putih with a percentage of 25%. The specific gene markers used in this study could distinguish the nucleotide variations that occurred between garlic-released varieties such as Lumbu Hijau, Lumbu Kuning, and Lumbu Putih and new accession such as Lumbu Hitam. The phylogenetic analysis showed that eleven local garlic accessions used in this study were separated into two main clusters. The first cluster consisted of eight accessions, while the second cluster consisted of three accessions. The markers used in this study would be promising to distinguish close genetic relationship accessions that could not be identified morphologically, especially to protect intellectual property in the future. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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