Antifungal Activity of Siderophore Isolated from Pantoea brenneri Against Fusarium oxysporum.

Autor: Suleimanova, A. D., Sokolnikova, L. V., Egorova, E. A., Berkutova, E. S., Pudova, D. S., Khilyas, I. V., Sharipova, M. R.
Zdroj: Russian Journal of Plant Physiology; Dec2023, Vol. 70 Issue 8, p1-7, 7p
Abstrakt: Iron is a ubiquitous element on Earth, but despite its abundance in the environment, it remains unavailable to many organisms. An antagonistic bacterium P. brenneri 3.5.1 was screened for the production of siderophores—iron-binding compounds on CAS agar and showed halo zones around the colonies. Bacteria secrete catechol-type siderophores at the concentration of 104 μM after 24 h of cultivation. The production of hydroxamate-type siderophores by P. brenneri 3.5.1 was not detected. The siderophore-containing metabolite fraction of P. brenneri 3.5.1 was extracted and purified. In an antagonistic plate assay, it inhibited the growth of Fusarium oxysporum mycelia in the absence of FeCl3. A complete cluster, containing 17 open reading frames, responsible for the production and export of an enterobactin-like siderophore, is present in the genome of P. brenneri 3.5.1. No other siderophore production genes or their homologues were found in the P. brenneri 3.5.1 genome. Therefore, taking together multiple PGP properties of P. brenneri 3.5.1 and after careful assessment of the biosafety of the strain, it could be considered as a biofungicide against Fusarium oxysporum. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index