| Abstrakt: |
In order to find out the status of plasma Heat shock Protein-72 during thermal and metabolic stress, ten each of periparturient Karan Fries (Karan Fries High yielder above 4500 kg/lactation and Low yielder below 3000kg/lactation) and Sahiwal cows (Sahiwal cows High yielder above 3000 kg/lactation and Low yielder below 2000kg/lactation) were selected during summer and winter season separately. Ten each of periparturient Karan Fries and Sahiwal cows during summer and winter season were selected separately. Further these animals were equally (5 each) divided into high and low producing based on the standard procedure and milk yield in earlier lactation the animals were selected. Blood samples from all the animals were collected from jugular vein using sterile heparinized vacutainer tubes during the morning hours from 8-9 am. before expected date of calving -45, -30, -15, 0 day of calving and 15, 30, and 45 after calving, posing minimum disturbance to animals during both the seasons, Plasma samples were analyzed for heat shock protein (HSP72) using radio-immunoassay diagnostic kits. Climatic variables (Tdb, Twb, Tmax, Tmin, RH and THI) were recorded on the day of blood collection. Plasma HSP72 levels were significantly (P<0.05) higher on the day of calving during summer season compared to winter season in Karan Fries cattle. The higher deviations in parameters of stress in high yielders compared to low yielder cows, suggest extra care must be given to high yielder animals. Based on the results of the present study, it can be concluded that the high yielding animals must be provided extra care and management including feeding during the periparturient period and extreme climatic conditions. [ABSTRACT FROM AUTHOR] |
