| Autor: |
Marri, Asadullah, Soomro, Aijaz Hussian, Shaikh, Nida, Irshad, Asif, Khan, Babar, Khatri, Umair, Gichki, Nisar Ahmed |
| Předmět: |
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| Zdroj: |
Pakistan Journal of Biotechnology; 2023, Vol. 20 Issue 2, p320-329, 10p |
| Abstrakt: |
The contamination of pathogenic microbes in street vended foods is a serious public health concern associated with foodborne illnesses (FBIs). The street vended Cholay Chat (SVCC) is a popular savory chickpea-based ready-to-eat food in Pakistan. The microbial quality of 18 SVCC samples was assessed through conventional and molecular identification. A total of three (03) bacterial species (i.e., Bacillus cereus, Macrococcus caseolyticus and Staphylococcus sciuri) were characterized via phenotypic and biochemical analysis in SVCC. Among them, B. cereus remained highly prevalent (88.88%) followed by M. caseolyticus (61.11%) and S. sciuri (11.11%). The polymerase chain reaction (PCR) based DNA amplification of the 16S rRNA gene and sequencing of isolated bacterial species were performed. The BLASTn analysis revealed that sequences were significantly aligned with B. cereus (97.38 %), M. caseolyticus (93.16%), and S. sciuri (97.21%). It is concluded that the microbial quality of SVCC is highly detrimental and thus is liable to cause FBIs among consumers. Therefore, safe food handling and manufacturing practices must be mandatorily assured by street food vendors to lessen the burden of FBIs among consumers. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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