Identification and experimental validation of ferroptosis-related gene lactotransferrin in age-related hearing loss.
| Autor: | Chaojun Zeng, Xi Gu, Yuqing Chen, Yanchun Lin, Junying Chen, Zhifeng Chen, Chenyu Chen, Guangnan Yao, Chang Lin |
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IN vitro studies
COCHLEA STAINS & staining (Microscopy) IN vivo studies ANALYSIS of variance PRESBYCUSIS ANIMAL experimentation MICROSCOPY WESTERN immunoblotting ONE-way analysis of variance BIOINFORMATICS T-test (Statistics) GENE expression profiling GLYCOSIDASES FLUORESCENT antibody technique DESCRIPTIVE statistics RESEARCH funding IRON overload DATA analysis software CELL death LIPID peroxidation (Biology) MICE DISEASE complications |
| Zdroj: | Frontiers in Aging Neuroscience; 2024, p1-11, 11p |
| Abstrakt: | Objective: To reveal the relationship between ARHL and ferroptosis and screen ferroptosis-related genes (FRGs) in ARHL. Methods: Bioinformatics were used to analyze the hub genes and molecular mechanism of ferroptosis in the aging cochleae. Senescence β-galactosidase staining, iron content detection, and micro malondialdehyde (MDA) assay kits were used to measure β-galactosidase activity, and expression of Fe2+ and MDA, respectively. Fluorescence microscope was used for immunofluorescence assay of hub genes. Western blot was used to verify the expression of hub genes in HEI-OC1 cells, cochlear explants, and cochleae of C57BL/6J mice. Data were expressed as mean ± SD of at least three independent experiments. Results: The analysis of bioinformatics confirmed that lactotransferrin (LTF) is the hub gene and CEBPA-miR-130b-LTF network is the molecular mechanism for cochlear ferroptosis. Compared with the control group, the experiments proved that the indicators of ferroptosis, including Fe2+, MDA, and LTF were differentially expressed in aging HEI-OC1 cells, aging cochlear explants, and aging cochleae. Conclusion: These results demonstrate that ferroptosis plays an important role in ARHL, and LTF is a potential therapeutic target for ARHL via regulating cochlear ferroptosis. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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