Autophagy and cell wall integrity pathways coordinately regulate the development and pathogenicity through MoAtg4 phosphorylation in Magnaporthe oryzae.

Autor: Guo, Pusheng, Wang, Yurong, Xu, Jiayun, Yang, Zhixiang, Zhang, Ziqi, Qian, Jinyi, Hu, Jiexiong, Yin, Ziyi, Yang, Leiyun, Liu, Muxing, Liu, Xinyu, Li, Gang, Zhang, Haifeng, Rumsey, Ryan, Wang, Ping, Zhang, Zhengguang
Předmět:
Zdroj: PLoS Pathogens; 1/30/2024, Vol. 20 Issue 1, p1-25, 25p
Abstrakt: Autophagy and Cell wall integrity (CWI) signaling are critical stress-responsive processes during fungal infection of host plants. In the rice blast fungus Magnaporthe oryzae, autophagy-related (ATG) proteins phosphorylate CWI kinases to regulate virulence; however, how autophagy interplays with CWI signaling to coordinate such regulation remains unknown. Here, we have identified the phosphorylation of ATG protein MoAtg4 as an important process in the coordination between autophagy and CWI in M. oryzae. The ATG kinase MoAtg1 phosphorylates MoAtg4 to inhibit the deconjugation and recycling of the key ATG protein MoAtg8. At the same time, MoMkk1, a core kinase of CWI, also phosphorylates MoAtg4 to attenuate the C-terminal cleavage of MoAtg8. Significantly, these two phosphorylation events maintain proper autophagy levels to coordinate the development and pathogenicity of the rice blast fungus. Author summary: Autophagy is a conserved cellular degradation and recycling process important in maintaining proper cellular homeostasis whereas the cell wall integrity (CWI) signaling pathway is also a conserved regulatory pathway in stress response. CWI signaling not only operates alone but also functions through crosstalk with autophagy during fungal infection of host plants. Despite the importance, little is known about how autophagy and CWI signaling coordinate their regulatory roles. Here, we have found that autophagy-related (ATG) protein MoAtg4 phosphorylation mediates the coordination between autophagy and CWI signaling, which underlies the development and pathogenicity of Magnaporthe oryzae. Both ATG protein MoAtg1 and CWI kinase MoMkk1 phosphorylate MoAtg4, which inhibits the deconjugation and C-terminal cleavage of MoAtg8, respectively, to maintain proper autophagy levels. Our study revealed a novel coordination between autophagy and CWI signaling and how the two pathways coordinate to regulate the development and pathogenicity of the blast fungus. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje