| Autor: |
Alves, Eric, Chopra, Abha, Ram, Ramesh, Currenti, Jennifer, Kalams, Spyros A., Mallal, Simon A., Phillips, Elizabeth J., Gaudieri, Silvana |
| Předmět: |
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| Zdroj: |
European Journal of Immunology; Jan2024, Vol. 54 Issue 1, p1-4, 4p |
| Abstrakt: |
Standard single-cell RNA-sequencing alignment pipelines often misassign killer immunoglobulin-like receptor (KIR) transcripts, leading to inaccuracies in quantifying KIR expression. This study found that activating KIR transcripts are frequently misclassified as inhibitory KIR expression, resulting in a skewed representation of the KIR repertoire. The misassignment is attributed to limitations in the analysis pipelines and the reference genomes used for alignment. The study suggests that realigning the data to a modified reference genome can improve the detection of KIR diversity, but challenges still exist due to the high homology among KIR genes. The findings highlight the need for caution when interpreting KIR expression in single-cell RNA-sequencing data and emphasize the importance of considering potential biases in reference genomes. [Extracted from the article] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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