Correlation between Peptacetobacter hiranonis , the baiCD Gene, and Secondary Bile Acids in Dogs.

Autor: Correa Lopes, Bruna, Chen, Chih-Chun, Sung, Chi-Hsuan, Ishii, Patricia Eri, Medina, Luis Fernando da Costa, Gaschen, Frederic P., Suchodolski, Jan S., Pilla, Rachel
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Zdroj: Animals (2076-2615); Jan2024, Vol. 14 Issue 2, p216, 11p
Abstrakt: Simple Summary: Dysmetabolism of bile acids has been linked to chronic enteropathy in dogs. Peptacetobacter (Clostridium) hiranonis has been described as the major species responsible for converting primary into secondary bile acids in dogs. Moreover, decreased P. hiranonis abundance has been linked to chronic enteropathy and antibiotic-induced dysbiosis in dogs and cats. Therefore, this study aimed to investigate further the correlation between P. hiranonis, the bacterial gene (baiCD) involved in bile acid conversion, and the conversion process per se. Our findings indicate a strong and significant correlation between P. hiranonis, baiCD, and the relative concentration of secondary bile acid in dogs. Bile acid metabolism is a key pathway modulated by intestinal microbiota. Peptacetobacter (Clostridium) hiranonis has been described as the main species responsible for the conversion of primary into secondary fecal unconjugated bile acids (fUBA) in dogs. This multi-step biochemical pathway is encoded by the bile acid-inducible (bai) operon. We aimed to assess the correlation between P. hiranonis abundance, the abundance of one specific gene of the bai operon (baiCD), and secondary fUBA concentrations. In this retrospective study, 133 fecal samples were analyzed from 24 dogs. The abundances of P. hiranonis and baiCD were determined using qPCR. The concentration of fUBA was measured by gas chromatography–mass spectrometry. The baiCD abundance exhibited a strong positive correlation with secondary fUBA (ρ = 0.7377, 95% CI (0.6461, 0.8084), p < 0.0001). Similarly, there was a strong correlation between P. hiranonis and secondary fUBA (ρ = 0.6658, 95% CI (0.5555, 0.7532), p < 0.0001). Animals displaying conversion of fUBA and lacking P. hiranonis were not observed. These results suggest P. hiranonis is the main converter of primary to secondary bile acids in dogs. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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