| Autor: |
Rodina, A. V., Zhirnik, A. S., Vysotskaya, O. V., Smirnova, O. D., Semochkina, Yu. P., Ratushniak, M. G., Cheshigin, I. V., Moskaleva, E. Yu. |
| Zdroj: |
Human Physiology; Dec2023, Vol. 49 Issue 7, p890-899, 10p |
| Abstrakt: |
The study was aimed at investigating the effect of low-dose rate (2.13 mGy/h) prolonged γ, n-irradiation at 0.05 and 0.5 Gy on the brain microglia, the expression of pro- and anti-inflammatory cytokine genes, the behavior and cognition of C57Bl/6 mice two months and one year after the exposure. The decrease in the number of resting microglia and the increase in the proportion of activated microglia in brain cell preparations were observed after γ, n-irradiation at a dose of 0.5 Gy. Immunohistochemical analysis revealed the increased number of microglial cells and the unaltered number of astrocytes in the hippocampus after γ, n-radiation at both doses. The expression of TNFα and IL-1β pro-inflammatory cytokine genes in the hippocampus increased after irradiation at both doses. The expression of TGFβ and IL-4 anti-inflammatory cytokine genes decreased two months after irradiation only at a dose of 0.5 Gy, suggesting more intensive neuroinflammation. The analysis performed a year later showed an age-related decrease in the number of resting microglia and an increase in the proportion of activated microglia compared to young animals, with more profound changes in male mice. The state of microglia in the control and irradiated mice was similar one year after the exposure. Impaired motor activity and spatial memory were detected in mice neither two months nor one year after irradiation; nevertheless, mice irradiated at a dose of 0.5 Gy demonstrated anxious behavior and decreased spatial learning in Morris water maze one year after the exposure. In the summary, we have shown that γ, n-irradiation at the doses of 0.05 and 0.5 Gy induced neuroinflammation two months after the exposure. No difference from the control mice was observed one year after irradiation. No disturbances of the behavior and spatial memory of irradiated mice were detected. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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