Effect of 21-Day Dry Immersion on Expression of Inborn Immunity Genes Associated with the Toll-Like Receptors of Signaling Pathways.

Autor: Vlasova, D. D., Sadova, A. A., Galina, V. S., Guermanov, N. S., Rykova, M. P., Antropova, E. N., Kutko, O. V., Shulguina, S. M., Orlova, K. D., Shmarov, V. A., Lysenko, E. A., Ponomarev, S. A.
Zdroj: Human Physiology; Dec2023, Vol. 49 Issue 7, p807-814, 8p
Abstrakt: Expression of inborn immunity genes was studied in 10 essentially healthy male subjects aged 24 to 32 years in a 21-day dry immersion (DI) experiment without countermeasures. To evaluate the humoral immunity function, we selected genes encoding Toll-like receptors; proteins mediating NF-kB, AP-1, Akt, and TLR4 pathways; apoptosis proteins; CLEC4E, HSPD1, and PTGS2 genes. Phenol-chloroform extraction was used to obtain total RNA from peripheral blood monocytes CD14+. Subsequently, total RNA was reverse transcribed to generate cDNA for real-time PCR. The results showed substantial changes in expression of these genes suggesting that changes in the TLR function on the transcriptional level are observed from the earliest stages of the immune response to 21-d DI. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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