The wound healing effect of polycaprolactone-chitosan scaffold coated with a gel containing Zataria multiflora Boiss. volatile oil nanoemulsions.
| Autor: | Osanloo, Mahmoud, Noori, Fariba, Varaa, Negar, Tavassoli, Alireza, Goodarzi, Aida, Moghaddam, Maryam Talebi, Ebrahimi, Lida, Abpeikar, Zahra, Farmani, Ahmad Reza, Safaei, Mohsen, Fereydouni, Narges, Goodarzi, Arash |
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| Předmět: |
PHYTOTHERAPY
INFLAMMATION prevention EDEMA prevention WOUND healing STATISTICS ESSENTIAL oils HERBAL medicine ANIMAL experimentation ONE-way analysis of variance POLYESTERS EMULSIONS FIBROSIS RATS COMPARATIVE studies DESCRIPTIVE statistics RESEARCH funding DATA analysis software DATA analysis TISSUE scaffolds |
| Zdroj: | BMC Complementary Medicine & Therapies; 1/25/2024, Vol. 24 Issue 1, p1-16, 16p |
| Abstrakt: | Aims: Thymus plant is a very useful herbal medicine with various properties such as anti-inflammatory and antibacterial. Therefore, the properties of this plant have made this drug a suitable candidate for wound healing. In this study, hydroxypropyl methylcellulose (HPMC) gel containing Zataria multiflora volatile oil nanoemulsion (neZM) along with polycaprolactone/chitosan (PCL-CS) nanofibrous scaffold was used, and the effect of three experimental groups on the wound healing process was evaluated. The first group, HPMC gel containing neZM, the second group, PCL-CS nanofibers, and the third group, HPMC gel containing neZM and bandaged with PCL-CS nanofibers (PCL-CS/neZM). Wounds bandaged with common sterile gas were considered as control. Methods: The nanoemulsion was synthesized by a spontaneous method and loaded into a hydroxypropyl methylcellulose (HPMC) gel. The DLS test investigated the size of these nanoemulsions. A PCL-CS nanofibrous scaffold was also synthesized by electrospinning method then SEM and contact angle tests investigated morphology and hydrophilicity/hydrophobicity of its surface. The animal study was performed on full-thickness skin wounds in rats, and the process of tissue regeneration in the experimental and control groups was evaluated by H&E and Masson's trichrome staining. Results: The results showed that the nanoemulsion has a size of 225±9 nm and has an acceptable dispersion. The PCL-CS nanofibers synthesized by the electrospinning method also show non-beaded smooth fibers and due to the presence of chitosan with hydrophilic properties, have higher surface hydrophobicity than PCL fibers. The wound healing results show that the PCL-CS/neZM group significantly reduced the wound size compared to the other groups on the 7th, 14th, and 21st days. The histological results also show that the PCL-CS/neZM group could significantly reduce the parameters of edema, inflammation, and vascularity and increase the parameters of fibrosis, re-epithelialization, and collagen deposition compared to other groups on day 21. Conclusion: The results of this study show that the PCL-CS/neZM treatment can effectively improve wound healing. [ABSTRACT FROM AUTHOR] |
| Databáze: | Complementary Index |
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