| Autor: |
Ravindran, Akila, Manivannan, Arun Chandra, Bharathi, Gunasekaran Sunandha Jeeva, Balasubramanian, Vellaisamy, Velmurugan, Palanivel, Sivasubramanian, Kanagasabapathy, Muruganandham, Moorthy, Arumugam, Natarajan, I. Almansour, Abdulrahman, Kumar, Raju Suresh, Sivakumar, Subpiramaniyam |
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| Zdroj: |
Biologia; Feb2024, Vol. 79 Issue 2, p605-619, 15p |
| Abstrakt: |
This study investigates the antibacterial activity of exopolysaccharides (EPS) produced by the marine bacterium Bacillus halotolerans against clinical strains of Pseudomonas aeruginosa and Serratia marcescens. EPS production was optimized by exploring different carbon sources with glucose, peptone, and calcium chloride identified as significant factors for enhanced production. The optimized medium achieved a production rate of 0.612 g/L, surpassing the unoptimized medium's production of 0.556 g/L. The EPS exhibited noteworthy hydroxyl scavenging activity and demonstrated hydrogen-donating ability by scavenging DPPH radicals. Fourier-transform infrared spectroscopy analysis revealed characteristic peaks representing hydroxyl and carboxyl groups, glycosidic linkages, and sulfate groups within the EPS structure. Nuclear magnetic resonance analysis confirmed the complex and heterogeneous nature of the EPS, with glucose and galactose residues and the presence of polysaccharides identified. Monomer units of the EPS structure were identified as glucose and galactose, and a prominent peak at 7.942 min indicated the presence of beta-1,5-O-Dibenzoyl-ribofuranose. This study provides valuable insights into optimizing EPS production, characterizing its properties, and evaluating its antibacterial activity against Pseudomonas sp. strains. Further investigations are necessary to explore the potential applications and underlying mechanisms of these antibacterial EPS, contributing to advancements in microbiology and biotechnology. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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