| Abstrakt: |
Mycoplasmosis is one of the economically important diseases in small ruminants. Mycoplasma affects respiratory system predominantly apart from synovial joints, udder and mucus membrane bearing surface. In the current study, a total of 514 samples consisting of 465 nasal swabs, 18 lung tissues, 16 milk, 11 synovial fluids, 3 ear swabs, and one fetal fluid were subjected for isolation of Mycoplasma in PPLO broth and PPLO agar. Inoculated samples were incubated at 37°C for 24-48 h under 5% CO2, until turbidity and colour change was observed. Broth cultures were also subjected for Mycoplasma genus specific and species specific PCR. Of the 514 samples that were cultured, 32 (6.22%) produced characteristic 'fried egg' microcolonies on the agar surface. Of the 32 isolates, 21 were M. ovipneumoniae, 7 were M. arginini and 4 unidentified mycoplasma species. Seventy five (14.59%) samples were positive for genus specific PCR. By species specific PCR, 21 (28%) samples were identified as M. ovipneumoniae, 13 (17.33%) M. arginini, whereas 41 (54.66) samples did not amplify with either of the clusters specific primers or other species specific primers and remain unidentified. [ABSTRACT FROM AUTHOR] |
