| Autor: |
Basirjafar, Pedram, Zandvakili, Raziyeh, Masoumi, Javad, Zainodini, Nahid, Taghipour, Zahra, Khorramdelazad, Hossein, Yousefi, Soheila, Tavakoli, Tayyebeh, Vatanparast, Mahboobeh, Safdel, Sepehr, Gheitasi, Mahsa, Ayoobi, Fatemeh, Naseri, Bahar, Jafarzadeh, Abdollah |
| Předmět: |
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| Zdroj: |
Immunopharmacology & Immunotoxicology; Feb2024, Vol. 46 Issue 1, p73-85, 13p |
| Abstrakt: |
In dendritic cells (DCs), leptin as an immune-regulating hormone, increases the IL-12 generation whereas it reduces the IL-10 production, thus contributing to TH1 cell differentiation. Using a murine model of breast cancer (BC), we evaluated the impacts of the Leptin and/or lipopolysaccharide (LPS)-treated DC vaccine on various T-cell-related immunological markers. Tumors were established in mice by subcutaneously injecting 7 × 105 4T1 cells into the right flank. Mice received the DC vaccines pretreated with Leptin, LPS, and both Leptin/LPS, on days 12 and 19 following tumor induction. The animals were sacrificed on day 26 and after that the frequency of the splenic cytotoxic T lymphocytes (CTLs) and TH1 cells; interferon gamma (IFN-γ), interleukin 12 (IL-12) and tumor growth factor beta (TGF-β) generation by tumor lysate-stimulated spleen cells, and the mRNA expression of T-bet, FOXP3 and Granzyme B in the tumors were measured with flow cytometry, ELISA and real-time PCR methods, respectively. Leptin/LPS-treated mDC group was more efficient in blunting tumor growth (p =.0002), increasing survival rate (p =.001), and preventing metastasis in comparison with the untreated tumor-bearing mice (UT-control). In comparison to the UT-control group, treatment with Leptin/LPS-treated mDC also significantly increased the splenic frequencies of CTLs (p <.001) and TH1 cells (p <.01); promoted the production of IFN-γ (p <.0001) and IL-12 (p <.001) by splenocytes; enhanced the T-bet (p <.05) and Granzyme B (p <.001) expression, whereas decreased the TGF-β and FOXP3 expression (p <.05). Compared to the Leptin-treated mDC and LPS-treated mDC vaccines, the Leptin/LPS-treated mDC vaccine was more effective in inhibiting BC development and boosting immune responses against tumor. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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