| Autor: |
BARAN, JOANNA, KURYK, ŁUKASZ, SZCZEPIŃSKA, TERESA, ŁAŹNIEWSKI, MICHAŁ, GAROFALO, MARIANGELA, MAZURKIEWICZ-PISAREK, ANNA, MIKIEWICZ, DIANA, MAZURKIEWICZ, ALINA, TRZASKOWSKI, MACIEJ, WIECZOREK, MAGDALENA, PANCER, KATARZYNA, HALLMANN, EWELINA, BRYDAK, LIDIA, PLEWCZYNSKI, DARIUSZ, CIACH, TOMASZ, MIERZEJEWSKA, JOLANTA, STANISZEWSKA, MONIKA |
| Předmět: |
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| Zdroj: |
Biotechnologia; 2023, Vol. 104 Issue 3, p403-419, 17p |
| Abstrakt: |
New prophylactic vaccine platforms are imperative to combat respiratory infections. The efficacy of T and B memory cell-mediated protection, generated through the adenoviral vector, was tested to assess the effectiveness of the new adenoviral-based platforms for infectious diseases. A combination of adenovirus AdV1 (adjuvant), armed with costimulatory ligands (ICOSL and CD40L), and rRBD (antigen: recombinant nonglycosylated spike protein rRBD) was used to promote the differentiation of T and B lymphocytes. Adenovirus AdV2 (adjuvant), without ligands, in combination with rRBD, served as a control. In vitro T-cell responses to the AdV1+rRBD combination revealed that CD8+ platform-specific T-cells increased (37.2 ± 0.7% vs. 23.1 ± 2.1%), and T-cells acted against SARS-CoV-2 via CD8+TEMRA (50.0 ± 1.3% vs. 36.0 ± 3.2%). Memory B cells were induced after treatment with either AdV1+rRBD (84.1 ± 0.8% vs. 82.3 ± 0.4%) or rRBD (94.6 ± 0.3% vs. 82.3 ± 0.4%). Class-switching from IgM and IgD to isotype IgG following induction with rRBD+Ab was observed. RNA-seq profiling identified gene expression patterns related to T helper cell differentiation that protect against pathogens. The analysis determined signaling pathways controlling the induction of protective immunity, including the MAPK cascade, adipocytokine, cAMP, TNF, and Toll-like receptor signaling pathway. The AdV1+rRBD formulation induced IL-6, IL-8, and TNF. RNA-seq of the VERO E6 cell line showed differences in the apoptosis gene expression stimulated with the platforms vs. mock. In conclusion, AdV1+rRBD effectively generates T and B memory cell-mediated protection, presenting promising results in producing CD8+ platform-specific T cells and isotype-switched IgG memory B cells. The platform induces protective immunity by controlling the Th1, Th2, and Th17 cell differentiation gene expression patterns. Further studies are required to confirm its effectiveness. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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