Genetic and biological signature of Potato Virus X circulating in Kazakhstan.

Autor: Azhimakhan, Moldir, Beisembina, Bibigul, Kapytina, Anastasiya, Kerimbek, Nazym, Gritsenko, Dilyara, Dianqiu Lv, Hu Baigeng, Khassanov, Vadim
Předmět:
Zdroj: Caspian Journal of Environmental Sciences; Dec2023, Vol. 21 Issue 5, p1151-1157, 7p
Abstrakt: This research studied ten isolates of potato virus X (PVX) isolated from potato cultivars growing in Kazakhstan (KZ), Belarus (BY) and China (CN). Additionally, the potato samples were screened for Potato virus Y (PVY), Potato virus S (PVS), and Potato leafroll virus (PLRV) using multiplex reverse transcription-polymerase chain reaction (RT-PCR). PVX was isolated from the potato varieties 'Tamasha', 'Red Scarlet', 'Gala variety', 'Krone', 'Juwel', 'Gala', and 'b2-7'. The identified isolates of PVX were sequenced and used to construct a phylogenetic tree, which demonstrated that KZ and BY isolates form clusters closely related to isolates from Asia and Europe, however, CN isolate formed a separate clade. KZ5 and KZ7 PVX isolates showed local symptoms of mosaic and light green speckled spots on the upper leaves of Datura stramonium in pathogenicity test. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index