The soil-borne white root rot pathogen Rosellinia necatrix expresses antimicrobial proteins during host colonization.

Autor: Chavarro-Carrero, Edgar A., Snelders, Nick C., Torres, David E., Kraege, Anton, López-Moral, Ana, Petti, Gabriella C., Punt, Wilko, Wieneke, Jan, García-Velasco, Rómulo, López-Herrera, Carlos J., Seidl, Michael F., Thomma, Bart P. H. J.
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Zdroj: PLoS Pathogens; 1/18/2024, Vol. 20 Issue 1, p1-32, 32p
Abstrakt: Rosellinia necatrix is a prevalent soil-borne plant-pathogenic fungus that is the causal agent of white root rot disease in a broad range of host plants. The limited availability of genomic resources for R. necatrix has complicated a thorough understanding of its infection biology. Here, we sequenced nine R. necatrix strains with Oxford Nanopore sequencing technology, and with DNA proximity ligation we generated a gapless assembly of one of the genomes into ten chromosomes. Whereas many filamentous pathogens display a so-called two-speed genome with more dynamic and more conserved compartments, the R. necatrix genome does not display such genome compartmentalization. It has recently been proposed that fungal plant pathogens may employ effectors with antimicrobial activity to manipulate the host microbiota to promote infection. In the predicted secretome of R. necatrix, 26 putative antimicrobial effector proteins were identified, nine of which are expressed during plant colonization. Two of the candidates were tested, both of which were found to possess selective antimicrobial activity. Intriguingly, some of the inhibited bacteria are antagonists of R. necatrix growth in vitro and can alleviate R. necatrix infection on cotton plants. Collectively, our data show that R. necatrix encodes antimicrobials that are expressed during host colonization and that may contribute to modulation of host-associated microbiota to stimulate disease development. Author summary: Most if not all organisms, including plants, associate with a wide diversity of microbes that live either inside these organisms, or in their immediate vicinity, and that collectively form their microbiota. Moreover, increasing evidence reveals that microbiota represent a key determinant for their health. To cause disease on their hosts, microbial pathogens need to overcome host immunity. Conceivably, pathogens need to overcome beneficial contributions that microbiota make to an organism's health. Here, we show that the genome of the fungal white root rot pathogen Rosellinia necatrix encodes putatively secreted antimicrobial proteins, many of which are expressed during plant colonization. Two of these proteins are functionally analyzed in this study, and we reveal that they can inhibit the growth of bacteria that antagonise R. necatrix growth in vitro and that can alleviate R. necatrix infection on cotton plants. Thus, we propose that R. necatrix employs antimicrobials during host colonization to promote host infection through the selective manipulation of host microbiota. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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