| Autor: |
Veenakumari, A. N., Arun, S. J., Sundareshan, S., Sheela, P., Bagalkote, Prashanth S., Rani, Kavitha, Mamatha, S. P. |
| Předmět: |
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| Zdroj: |
Journal of Experimental Zoology India; Jan2024, Vol. 27 Issue 1, p1103-1112, 10p |
| Abstrakt: |
The current study was undertaken to identify Bovine Rotavirus (BRV) and Bovine Coronaviruses (BCoV) in the faeces of both diarrheal and non-diarrheic calves. The BRV and BCoV were detected by the reverse transcriptase polymerase chain reaction (RT-PCR) technique using appropriate primers. A total of 162 faecal samples from young calves (below six months of age), with and without diarrhoea, were obtained for this study. The BRV and BCoV were identified by performing RTPCR to all the 162 faecal samples by focusing on the VP6 and N genes, respectively. The BRV was genotyped by P-genotyping wherein RT-PCR was performed for the rotavirus positive samples (VP6 gene positive samples) and VP4 gene was targeted. In both diarrheal and non-diarrheic calf faecal samples, bovine rotavirus was found in 47.53% (77 out of 162), but none of the faecal samples tested were positive for BCoV. The BRV was genotyped by P-genotyping wherein RT-PCR was performed for the rotavirus positive samples (VP6 gene positive samples) and VP4 gene was targeted. The P-genotyping demonstrated that 83.11% (64/77) of the samples were positive and possessed VP4 gene. In total, 77 samples (47.53%) were found to be positive for BRV from diarrheal calves between the ages of 1-4 months. Out of these positive samples, 83.11% (64/77) of the samples were belonging to the P-genotype having VP4 gene. However, BCoV infection was not identified in the present study. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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