| Autor: |
Borschevskaya, L. N., Gordeeva, T. L., Pichkur, E. B., Samygina, V. R., Sineoky, S. P. |
| Předmět: |
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| Zdroj: |
Applied Biochemistry & Microbiology; Dec2023, Vol. 59 Issue 9, p1132-1139, 8p |
| Abstrakt: |
Successful assembly of bacteriophage MS2 virus-like particles (VLPs) from a secreted recombinant capsid protein in the culture medium of the Komagataella phaffii methylotrophic yeast has been shown. The yield of VLPs was 5 g/L and reached 30% of the total protein of the culture liquid supernatant. The VLPs were well-ordered icosahedral structures with a diameter of 26‒28 nm, which was confirmed by transmission electron microscopy. A simple method for the isolation and purification of the VLPs was proposed, which ensures the purity of the target protein structures of more than 90%. The results we obtained can be used for development of a technology for the large-scale production of recombinant vaccines based on bacteriophage MS2 VLPs. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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