| Abstrakt: |
Introduction: Osteoarthritis is a progressive, age-related disease characterized by cartilage destruction, subchondral bone formation, and inflammation of the synovial membrane. Given the side effects of conventional OA treatments, including nonsteroidal anti-inflammatory drugs and corticosteroids, it is important to consider new treatment options for this disorder. Recently, the role of matrix metalloproteinase in the pathogenesis of osteoarthritis has attracted attention. Objective: The aim of this study was to investigate the effect of Satureja sahendica Bornm essential oil on the expression of matrix metalloproteinases 1, 2, 9 and 13 in a model resembling osteoarthritis. Material & Methods: Bovine fibroblast-like synoviocytes were isolated from the articular cartilage of the radiocarpal joint of an 8-month-old Holstein bovine. Using RT-PCR and ELISA . the expression of Matrix metalloproteinase 1, 2, 9, and 12 was measured. The effect of Satureja sahendica Bornm essential oil on migration and cell invasion was investigated in transwell. Results: The results showed that Satureja sahendica Bornm essential oil significantly reduced the expression of matrix metalloproteinase 9 and 12 in bovine fibroblast-like synoviocytes stimulated by lipopolysaccharide. It also suppresses the migration and invasion of these cells. However, the essential oil of Satureja sahendica Bornm had no significant effect on the expression of matrix metalloproteinase 1 and 2. Discussion & Conclusion: Our results show that the essential oil of Satureja sahendica Bornm can significantly reduce the activity and inflammatory effect of matrix metalloproteinase 9 and 12. Its potential role as an adjunct to current treatments for osteoarthritis was confirmed. However, cellular modeling does not confirm its beneficial effect in patients with osteoarthritis. [ABSTRACT FROM AUTHOR] |
