| Abstrakt: |
Simple Summary: Temperature is a pervasive environmental factor that plays a crucial role in the lives of aquatic organisms. However, the mechanisms governing the response to temperature remain unclear in teleost fish. In this research, the marine benthic fish Chinese tongue sole (Cynoglossus semilaevis), which is economically significant in northern China, is used as a model to analyze this issue. The brains of female and male C. semilaevis were examined via transcriptomics technology to investigate their response to temperature. Our findings revealed the existence of specific genes and pathways involved in cortisol synthesis and secretion, neuroactive ligand–receptor interactions, TGF-beta signaling pathway, and JAK/STAT signaling pathway. The genes identified includes the HSP family, tshr, c-fos, c-jun, cxcr4, camk2b, and igf2. Our study provides some evidence that C. semilaevis responds to temperature, highlighting a potential path for further investigation. As a common influencing factor in the environment, temperature greatly influences the fish that live in the water all their life. The essential economic fish Chinese tongue sole (Cynoglossus semilaevis), a benthic fish, will experience both physiological and behavioral changes due to increases in temperature. The brain, as the central hub of fish and a crucial regulatory organ, is particularly sensitive to temperature changes and will be affected. However, previous research has mainly concentrated on the impact of temperature on the gonads of C. semilaevis. Instead, our study examines the brain using transcriptomics to investigate specific genes and pathways that can quickly respond to temperature changes. The fish were subjected to various periods of heat stress (1 h, 2 h, 3 h, and 5 h) before extracting the brain for transcriptome analysis. After conducting transcriptomic analyses, we identified distinct genes and pathways in males and females. The pathways were mainly related to cortisol synthesis and secretion, neuroactive ligand–receptor interactions, TGF beta signaling pathway, and JAK/STAT signaling pathway, while the genes included the HSP family, tshr, c-fos, c-jun, cxcr4, camk2b, and igf2. Our study offers valuable insights into the regulation mechanisms of the brain's response to temperature stress. [ABSTRACT FROM AUTHOR] |
