Bioinformatic Approach of B and T Cell Epitopes of PLD and CP40 Proteins of Corynebacterium pseudotuberculosis ovis Mexican Isolate 2J-L towards a Peptide-Based Vaccine.

Autor: Rodríguez-Domínguez, Maria Carla, Montes-de-Oca-Jiménez, Roberto, Vázquez-Chagoyán, Juan Carlos, Rivadeneira-Barreiro, Pilar Eliana, Zambrano-Rodríguez, Pablo Cleomenes, Ruiz-Riva-Palacio, Martha Elba, Gutiérrez-Castillo, Adriana del Carmen, de-Castro-Soares, Siomar, Vieyra-Reyes, Patricia, Arteaga-Troncoso, Gabriel
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Zdroj: International Journal of Molecular Sciences; Jan2024, Vol. 25 Issue 1, p270, 19p
Abstrakt: Mapping B and T cell epitopes constitutes an important action for peptide vaccine design. PLD and CP40 virulence factors of Corynebacterium pseudotuberculosis biovar ovis, a causal agent of Caseous Lymphadenitis, have been evaluated in a murine model as good candidates for vaccine development. Therefore, the goal of this work was to in silico analyze B and T cell epitopes of the PLD and CP40 proteins of a Mexican isolate of Corynebacterium pseudotuberculosis ovis. The Immune Epitope Data Base and Resource website was employed to predict the linear and conformational B-cell, T CD4+, and T CD8+ epitopes of PLD and CP40 proteins of Corynebacterium pseudotuberculosis ovis Mexican strain 2J-L. Fifty B cell epitopes for PLD 2J-L and forty-seven for CP40 2J-L were estimated. In addition, T CD4+ and CD8+ cell epitopes were predicted for PLD 2J-L (MHC I:16 epitopes, MHC II:10 epitopes) and CP40 2J-L (MHC I: 15 epitopes, MHC II: 13 epitopes). This study provides epitopes, paying particular attention to sequences selected by different predictor programs and overlap sequences as B and T cell epitopes. PLD 2J-L and CP40 2J-L protein epitopes may aid in the design of a promising peptide-based vaccine against Caseous Lymphadenitis in Mexico. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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