Development of a Predictive Model for Optimization of Embryo Transfer Timing Using Blood-Based microRNA Expression Profile.

Autor: Chen, Ming-Jer, Hsu, An, Lin, Pei-Yi, Chen, Yu-Ling, Wu, Ko-Wen, Chen, Kuan-Chun, Wang, Tiffany, Yi, Yu-Chiao, Kung, Hsiao-Fan, Chang, Jui-Chun, Yang, Wen-Jui, Lu, Farn, Guu, Hwa-Fen, Chen, Ya-Fang, Chuan, Shih-Ting, Chen, Li-Yu, Chen, Ching-Hung, Yang, Pok Eric, Huang, Jack Yu-Jen
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Zdroj: International Journal of Molecular Sciences; Jan2024, Vol. 25 Issue 1, p76, 11p
Abstrakt: MicroRNAs (miRNAs) can regulate the expression of genes involved in the establishment of the window of implantation (WOI) in the endometrium. Recent studies indicated that cell-free miRNAs in uterine fluid and blood samples could act as alternative and non-invasive sample types for endometrial receptivity analysis. In this study, we attempt to systematically evaluate whether the expression levels of cell-free microRNAs in blood samples could be used as non-invasive biomarkers for assessing endometrial receptivity status. We profiled the miRNA expression levels of 111 blood samples using next-generation sequencing to establish a predictive model for the assessment of endometrial receptivity status. This model was validated with an independent dataset (n = 73). The overall accuracy is 95.9%. Specifically, we achieved accuracies of 95.9%, 95.9%, and 100.0% for the pre-receptive group, the receptive group, and the post-respective group, respectively. Additionally, we identified a set of differentially expressed miRNAs between different endometrial receptivity statuses using the following criteria: p-value < 0.05 and fold change greater than 1.5 or less than −1.5. In conclusion, the expression levels of cell-free miRNAs in blood samples can be utilized in a non-invasive manner to distinguish different endometrial receptivity statuses. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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