| Autor: |
Bagasariya, Deepkumar, Charankumar, Kondasingh, Shah, Saurabh, Famta, Paras, Fernandes, Valencia, Shahrukh, Syed, Khatri, Dharmendra Kumar, Singh, Shashi Bala, Srivastava, Saurabh |
| Předmět: |
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| Zdroj: |
Journal of Microencapsulation; Jan2024, Vol. 41 Issue 1, p27-44, 18p |
| Abstrakt: |
Our aim was to repurpose atorvastatin for melanoma by encapsulating in a nanostructured lipid carrier matrix to promote tumour cell internalisation and skin permeation. pH-responsive chitosan gel was employed to restrict At-NLCs in upper dermal layers. We utilised a quality by design approach for encapsulating At within the NLC matrix. Further, cellular uptake and cytotoxicity was evaluated along with pH-responsive release and ex vivo skin permeation. Cytotoxicity assay showed 3.13-fold enhanced cytotoxicity on melanoma cells compared to plain drug with nuclear staining showing apoptotic markers. In vitro, release studies showed 5.9-fold rapid release in chitosan gel matrix at pH 5.5 compared to neutral pH. At-NLCs prevented precipitation, promoted skin permeation, and SK-MEL 28 cell internalisation. The localisation of NLCs on the upper dermal layer due to electrostatic interactions of skin with chitosan gel diminished the incidence of untoward systemic effects. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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