| Abstrakt: |
The plastic additive bisphenol S (BPS) has been widely used in various industrial and household products, and frequently detected in environmental and human samples. For the past few years, increasing concern has been focused on the visual toxicity of BPS. Here, after zebrafish embryos were exposed to BPS (1, 10, 100, and 1 000 μg·L-1) for 15 days, retinal histology and expression of genes involved in retinoic acid metabolism and opsin genes were examined to investigate the effect of BPS on the early visual development of larval zebrafish. The results showed that exposure to 10, 100 and 1 000 μg·L-1 BPS inhibited the expression of genes related to retinoic acid synthesis and the expression of neural retina leucine zipper gene (nrl) that related to photoreceptor cell development, while the expression of opsin genes (zfblue, zfuv and zfgr) was increased in 100 μg·L-1 and 1 000 μg· L-1 BPS groups. The results of hematoxylin-eosin staining showed that the thickness of the retina, the ganglion cell layer, the inner nuclear layer, and the outer nuclear layer was significantly decreased by different doses of BPS. In addition, BPS also induced small eyes in 15-dpf zebrafish larvae, based on Pearson correlation analysis of the body length, the head width, and the eye diameter. In summary, our study suggested that BPS probably affected early retinal development of zebrafish by interfering with retinoic acid metabolism, and its potential health risk to the visual system should arouse extensive attention. [ABSTRACT FROM AUTHOR] |
