| Autor: |
Kang, Guiyu, Jiao, Yang, Pan, Pan, Fan, Huiping, Li, Qiang, Li, Xiangying, Li, Jingtan, Wang, Yan, Jia, Yanfei, Wang, Jingting, Sun, Haiji, Ma, Xiaoli |
| Předmět: |
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| Zdroj: |
Carcinogenesis; Oct/Nov2023, Vol. 44 Issue 10/11, p773-784, 12p |
| Abstrakt: |
Objectives The CHRNΑ5 gene, which encodes the α5-nicotinic acetylcholine receptor (α5-nAChR), is related to lung cancer and nicotine addiction. Smoking is closely related to the immunosuppressive effect of macrophages. CD47, a phagocytosis checkpoint in macrophages, is a therapeutic target in various cancer types. Nevertheless, the relationship between α5-nAChR and CD47 in lung cancer is still unclear. Methods and Results The present study showed that α5-nAChR-mediated CD47 expression via STAT3 signaling, consequently leading to tumor progression and immune suppression in lung adenocarcinoma (LUAD). α5-nAChR expression was correlated with STAT3 expression, CD47 expression, smoking status and poor prognosis of LUAD in vivo. In vitro , α5-nAChR expression mediated the phosphorylation of STAT3, and phosphorylated STAT3 bound to the CD47 promoter and mediated CD47 expression. Downregulation of α5-nAChR and/or CD47 significantly reduced cell proliferation, migration, invasion, stemness and IL-10 expression, but increased TNF-α expression and phagocytosis of macrophages in LUAD. Furthermore, α5-nAChR/CD47 signaling contributed to the growth of subcutaneous xenograft tumors and liver metastasis of tumors in mice. Conclusion The α5-nAChR/STAT3/CD47 axis contributed to the progression and immune escape of lung cancer and may be a potential target for LUAD immunotherapy. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
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