| Abstrakt: |
Objective: To evaluate the effect of treatment, temperature, and time on 2-mm long stems of Solanum quitoense, using the minimal growth technique, under in vitro conditions. Design/Methodology/Approach: Eight treatments with different concentrations of mannitol, sucrose, and Murashige and Skoog (1962) (0 5g L-1, 10 g L-1, 15 g L-1, 20 g L-1, 25 g L-1, 30 g L-1, and 30 g L-1) were analyzed. The experiments were placed in two rooms at 25 °C and 21 °C. Stem growth was recorded every fifteen days. Results: The Generalized Linear Model showed that the treatments with the best results were 20 g L-1 and 30 g L-1 mannitol, which reduced the in vitro growth of S. quitoense to a remarkable degree, preserving the subsistence and vigor characteristics, at a temperature of 21 °C. meanwhile the applied concentrations of sucrose promoted a rapid growth of both the stem and shoots. Findings/Conclusions: S. quitoense recorded resistance to 30 g L-1 mannitol, enabling a 3-month preservation of seedlings; however, S. quitoense could potentially be preserved for longer periods. [ABSTRACT FROM AUTHOR] |
