| Autor: |
Chairunisa, Rachmat, Agus, Perdani, Ambar Yuswi, Sulistyowati, Yuli, Herliana, Lina, Roy, Stuart J. |
| Předmět: |
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| Zdroj: |
AIP Conference Proceedings; 2023, Vol. 2972 Issue 1, p1-8, 8p |
| Abstrakt: |
Rice is one of the world's most consumed food crops, and productivity is significantly affected by environmental conditions, such as soil salinity. Due to the gradual rising of sea level, the development of salinity-tolerant rice is crucial to maintain food security, especially in coastal zones. Two homozygous transgenic rice lines (IR411-17-10-2 and IR411-19-06-1) harboring the OsActin::AtAVP1 gene were evaluated for their salinity tolerance during the vegetative stage by measuring the projected shoot area (PSA) and the leaf K+/Na+ ratio under normal and salt stress (120 mM NaCl) conditions. To overcome the laborious and time-consuming nature of conventional measurement, the plant's shoot area was measured automatically using the LemnaTec 3D Scanalyzer system. The basic principle of this image-based phenotyping is the conversion of shoot image pixels into Projected Shoot Area (PSA) by LemnaTec Grid software. The phenotyping was done for 28 days. Non-transgenic IR64 and a null line were also grown as negative controls for comparison. Under normal and salt conditions, the projected shoot area of IR411-17-10-2 was similar to both controls, while the projected shoot area of IR411-19-06-1 was lower than both controls. Another common parameter for salt tolerance is leaf ionic ratio (K+/Na+). The K+/Na+ ratio of transgenic lines was compared to IR64 and null controls to see any significant differences. The K+/Na+ ratio of both transgenic lines was similar to that of IR64 and null controls under normal and salt stress conditions. These findings may indicate that constitutive expression of AtAVP1 in rice c.v IR64 does not have any significant effects on the plant's performance and salt tolerance at the early vegetative stage. These findings should be further validated by gene expression analyses before any firm conclusion can be drawn. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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