| Abstrakt: |
The vetiver oil production in Indonesia decreases yearly due to its low quality, so it is necessary to develop superior vetiver plants through in vitro mutagenesis. This research aimed to evaluate the gamma-ray irradiation effect on vetiver plantlet regeneration and acclimatization. The in vitro shoots were irradiated with gamma-rays at doses of 0, 15, 30, 45, 60, and 75 Gy. Irradiated shoots were multiplied and regenerated into plantlets on culture medium, then acclimatized on the soil growing medium. The results showed that gamma-ray irradiation on shoots culture inhibited plantlet regeneration and acclimatization but did not significantly affect plantlet height growth. Inhibition in the plantlet regeneration capacity of irradiated shoots occurred at high irradiation doses (45-75 Gy). The plantlet regeneration capacity of non-irradiated and irradiated shoots at low doses (15-30 Gy) was 100%, while at high doses (45-75 Gy), it was 72-92%; regeneration capacity decreased with increasing irradiation doses. Plantlets regenerated from non-irradiated and irradiated shoots had good acclimatization survival (100%). The survival frequency decreased (80%) at the acclimatization stage only occurred at a high dose of 75 Gy. [ABSTRACT FROM AUTHOR] |
