The role of extractives in the natural durability of the heartwood of Dicorynia guianensis Amsh: new insights in antioxydant and antifungal properties.

Autor: Say Anouhe, Jean-Baptiste, Bobelé Niamké, Florence, Faustin, Milcard, Virieux, David, Pirat, Jean-Luc, Amissa Adima, Augustin, Kati-Coulibaly, Seraphin, Amusant, Nadine
Zdroj: Annals of Forest Science (BioMed Central); Mar2018, Vol. 75 Issue 1, p1-10, 10p, 1 Diagram, 5 Charts, 1 Graph
Abstrakt: Context The heartwood of Dicorynia guianensis Amsh is resistant to white rot fungi decay, but the mechanism of this natural durability is not fully elucidated. Aims Biochemical studies were carried out in order to better understand the role of extractives in natural durability of D. guianensis. Methods The powders from durable and moderately durable heartwood were extracted with methanol, ethanol, and hot water. The quantity of total phenols, tannins, and flavonoids as well as antioxidant activity, evaluated by 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) were determined using colorimetric methods. Antifungal activity was assessed by using two white rot fungi. The bioactive fractions and compounds were obtained using bio-guided fractionation, HPLC isolation, MS and RMN spectroscopic analyses. Results Durable woods contain higher amounts of heartwood extract and antioxidant activity. Antioxidant activity was highly correlated with the content of phenolics. The purification of the most antioxidant fraction FII affords the characterization of (+)-catechin (−)-epicatechin, neoastilbin, astilbin, and isoastilbin. Alkaloid fraction FIII exhibits dose-dependent fungistatic activity against Pycnoporus sanguineus Linnaeus and Trametes versicolor Quelet. Conclusion Phenolic antioxidants and fungistatic alkaloids positively impact the natural durability of D. guianensis. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index