Assessment of the Biocontrol Potential of Entomopathogenic Fungi against The Red Spider Mite Tetranychus urticae.

Autor: RANADEV, P., NAGARAJU, K., MUTHURAJU, R., KUMAR, R. VASANTH
Předmět:
Zdroj: Mysore Journal of Agricultural Sciences; Oct-Dec2023, Vol. 57 Issue 4, p231-239, 9p
Abstrakt: Entomopathogenic fungi (EPF) are the most versatile and potential biocontrol agents, due to their adaptability, mode of entry, persistence nature and wide host range. The present study aimed to isolate, identify, and evaluate the virulence of entomopathogenic fungi (EPF) for their potential as biocontrol agents against red spider mites (Tetranychus urticae). Out of 81 fungal isolates, 16 (19.25%) showed insecticidal activity under in vitro conditions. The results revealed that among 16 isolates, ENPF-8 and ENPF-58 had significantly higher mortality rates (93-95 %) at a concentration of 1x108 conidia/ml after 9 days after treatment (DAT). The virulent fungal isolates were identified as Isaria fumosorosea (Gene bank accession number: MT997932) and Hirsutella thompsonii (Gene bank accession number: MT997936) by sequencing the ITS region of the 18S rRNA gene. H. thompsonii (ENPF-58) was found to be more virulent against test insects, with lower LC50 (7.6x105 conidia/ml) and LT50 (5.7, 5.89 and 5.99 days) values compared to the other EPF. Based on the results, H. thompsonii could be a promising biocontrol agent with a wide host range and high virulence as tested in other experiments. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index