In-vitro Screening of Actinobacteria Isolated from Natural Ecosystem for Production of Hydrolytic Enzymes.

Autor: BINDUSHREE, C., SHIVAPRAKASH, M. K., NAIK, L. KRISHNA
Předmět:
Zdroj: Mysore Journal of Agricultural Sciences; Oct-Dec2023, Vol. 57 Issue 4, p68-75, 8p
Abstrakt: Actinobacteria are considered as a promising source for novel bioactive compounds and hydrolytic enzymes with a broad range of biological activities. Isolation and screening of actinobacteria for hydrolytic enzyme production was carried out and a total of one hundred and twenty isolates were obtained from different ecosystem like forest soil, compost and rhizosperic soils of vegetable crops. Out of 120 isolates, 39 showed amylase activity with dissolution factor varied from 0.10 - 3.50. Twenty five isolates exhibited cellulase activity with the dissolution factor ranged from 0.10-3.50. The actinobacteria isolate CA32 from compost showed maximum cellulolytic activity with dissolution factor of 3.50. Twenty one actino bacterial isolates showed chitinolytic activity ranging from 0.10-2.00 and maximum dissolution factor was recorded by isolate of rhizosperic soil SA12 (2.00) followed by the isolate of compost CA4 (1.43). Twenty six isolates exhibited lipolytic activity with the dissolution factor ranging from lowest 0.11 in SA22 to highest 1.64 in FA12. The hydrolysis of complex organic compounds by actinobacterial isolates in-vitro showed that the actinobacteria are good degraders of organic matter in the soil. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index