Autor: |
Kim, Hyeyun, Pyo, Ju Yeon, Moon, Jiyeon, Lee, Seungeun, Kim, Minchae, Choi, Yein, Shin, Dong-Ick, Park, Byong-Gon |
Zdroj: |
Molecular & Cellular Toxicology; Oct2023, Vol. 19 Issue 4, p789-798, 10p |
Abstrakt: |
Background: Obstructive sleep apnea (OSA) is a common disorder that is associated with increased cerebrovascular disease and neurocognitive impairment. Although reports on cardiovascular and cerebrovascular morbidity have been conducted, there are few research on the mechanism related to cognitive decline and dementia especially, Alzheimer's disease (AD). Recent study reinforces OSA-AD link with amyloid deposition in the OSA brain and APOE genotype prevalence in OSA. This study is designed to find a link between OSA and neurodegenerative with successful animal model setting. Methods: A total of 16 rats were used, divided into the control and OSA group. For OSA, 0.3% cross-linked hyaluronic acid was injected twice every 12 weeks into the base of the tongue to create an OSA model. After 12 and 24 weeks, chronic OSA was identified with full channel polysomnography (PSG). The Morris water maze (MWM) test was conducted in control and OSA groups at 22 weeks, and pathological findings were subsequently confirmed 24 weeks after OSA induction. In addition, we studied the epigenetic changes with miRNA to identify the biomarkers for the prediction of dementia in OSA. Results: In the MWM test, the speed of finding the platform was lower than that of the control group (47.0 ± 13.9 s; OSA group/12.4 ± 6.1 s; control, p < 0.01). In brain histopathologic changes, disorganized cortex layers in OSA group were prominent compared with the control cortex. Hippocampal cortex in OSA also showed a disorganized CA1 region with degenerative neurons and fibrillary changes, compared with the control. The miRNA analysis identified an up-regulation in MiR-132-5p/137-3p/137-5p/501-3p, also known as AD, and a down-regulation of MiR-182/183-3p/183-5p/200a-3p/200b-3p/21-5p. Conclusion: OSA rat model with tongue hypertrophy showed neurodegenerative changes in brain and the epigenetic changes of mRNA/miRNA which have been known as AD-related genes. [ABSTRACT FROM AUTHOR] |
Databáze: |
Complementary Index |
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