| Autor: |
Thuy-Dung Doan-Tran, Thanh-Tan Nguyen, Hieu Tran-Van |
| Předmět: |
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| Zdroj: |
Jordan Journal of Biological Sciences; Dec2023, Vol. 16 Issue 4, p699-704, 6p |
| Abstrakt: |
Immobilizing antibodies onto the surface of gold nanoparticles (AuNPs) in an oriented manneris essential to achieve high sensitivity and detection limits in immunoassays and biosensors. In this research, the two-domain protein proAx1-Au-binding was generated as the result of the fusion between staphylococcalprotein A and a gold-binding peptide (GBP). This novel protein allows the fragment antigen-binding (Fab) region of captured antibodies to be exposed while binding stably to the gold surface. In this present study, we successfully constructed the recombinant pET22b-proAx1-Au-binding plasmid. The expression of proAx1-Au-binding was induced by Isopropyl β-d-1-thiogalactopyranoside (IPTG) and then confirmed by Tricine-SDS-PAGE and Western Blot.The gold-binding ability of this novel protein was evaluated and the result showed that proAx1-Au-binding had a higher affinity for AuNPs than protein A alone, thereby enhancing antibody immobilization onto AuNPs. This study laid the groundwork for facilely preparing any target-specific antibody-AuNPs. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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