| Autor: |
Angela, Snavely, Corbin D., Adair-Hudson, Blakely M., Vasicek, Thaddeus W. |
| Zdroj: |
Chemist; Dec2023, Vol. 94 Issue 2, p1-13, 13p |
| Abstrakt: |
The immobilization of enzymes to magnetic nanoparticles facilitates the recovery and reuse of the enzyme, which mitigates the financial burden of enzyme use. Particles are typically modified to enhance enzyme immobilization. However, the modification of magnetic nanoparticles alters the magnetic properties of the particles which may diminish the particle recovery and therefore decrease the efficiency of magnetically recycled catalysts. In this work, magnetic nanoparticles were synthesized and modified with varying amounts of an aminosilane. β-Glucosidase, an enzyme relevant for bioethanol production, was immobilized on the particles. The enzyme immobilized particles were subjected to a recycling experiment, where the activity and particle loss during recycling were quantified. The particle loss was ~2% × cycle-1 and was not dependent upon the extent of aminosilane modification. The activity loss × cycle-1 was ~3% and did not depend on the extent of aminosilane modification but was proportional to the particle loss. The results from this study suggest that increasing the recovery of the magnetic nanoparticles will decrease the loss of recycled enzyme activity. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
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