| Abstrakt: |
Researchers at the Affiliated Hospital of Youjiang Medical University for Nationalities in Guangxi, China, have developed a new method for detecting Escherichia coli (E.coli) using electrochemical biosensing. The traditional method of using antibodies for detection is often complex and prone to false positives. The researchers replaced antibodies with nucleic acid aptamers and utilized triple-helical DNA to inhibit the cleavage activity of the CRISPR/Cas gene editing system, enabling high-specificity detection of E.coli. The method demonstrated high sensitivity, low detection limits, and high specificity, making it a promising approach for analyzing E.coli and potentially other pathogenic bacteria. [Extracted from the article] |
