Abstrakt: |
Dunaliella salina is a carotenoid-producing microalgae that is important for its extraordinary ability to accumulate ß-carotene. Therefore, an experiment was performed to investigate the effect of different concentrations of gibberellic acid on growth, accumulation of chlorophyll pigments, carotenoid content, and dry biomass of D. salina in f/2 medium. Experimental treatments included different concentrations of gibberellic acid (GA3): 0 (control), 0.5, 5, 10, and 20 mg/L. The measured traits in this experiment included growth rate and doubling time, chlorophyll a, chlorophyll b, total chlorophyll, total carotenoids, dry biomass, total lipid content, and endogenous gibberellic acid. Analysis of variance showed that the effects of different concentrations of gibberellic acid on all studied traits of D. salina were significant (P≤ 0.05). Gibberellic acid (10 mg/l) increased growth rate, chlorophyll a and b contents, total chlorophyll content, dry biomass, and total lipid content in the studied microalgae while the highest level of total carotenoid accumulation was recorded with 5 mg/L GA3 treatment. According to the findings, it is suggested that GA3 5 mg/L can be effective when the purpose of microalgae cultivation is to produce carotenoids, but if the aim of production is dry biomass and total lipid contents, application of 10 mg/L GA3 is recommended to stimulate and increase the production of microalgae total lipid and dry biomass. [ABSTRACT FROM AUTHOR] |