Kinetics and Mechanisms of Oxidative Hemolysis of Erythrocytes under the Action of Azo- and Peroxide Initiator.

Autor: Sokolova, E. M., Dubenskaia, N. A., Psikha, B. L., Neshev, N. I.
Zdroj: Biophysics; Aug2023, Vol. 68 Issue 4, p570-575, 6p
Abstrakt: The kinetics of oxidative hemolysis of the 0.2% mouse erythrocytes suspension has been studied over a wide range of concentrations under the action of radical-forming initiators 2,2'-azobis(2-amidinopropan) dihydrochloride and tert-butyl hydroperoxide. Hemolysis of erythrocytes proceeded upon activation lipid peroxidation in erythrocyte membranes. In the case of tert-butyl hydroperoxide, oxidative processes also developed in the soluble part of the cell, leading to the formation of insoluble hemoglobin aggregates. Period of hemolysis induction (time of reaching 10% hemolysis) under the influence of 2,2'-azobis(2-amidinopropane) dihydrochloride decreases inversely to the square root of the initiator concentration, which is consistent with the classical theory of radical chain oxidation of hydrocarbons. In the case of tert-butyl hydroperoxide this pattern is not observed. In the erythrocyte model of testing natural and synthetic compounds for antioxidant activity it is preferable to use 2,2'-azobis (2-amidinopropane) dihydrochloride as the initiator of lipid peroxidation. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
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