THE MANOMETRIC ESTIMATION OF GLUTATHIONE IN TISSUE EXTRACTS.

Autor: Ennor, A. H.
Zdroj: Australian Journal of Experimental Biology & Medical Science; Jun1939, Vol. 17 Issue 2, p157-172, 16p, 15 Charts, 3 Graphs
Abstrakt: A modified manometric technique for the estimation of GSH in rat and mouse liver extracts has been described. The difficulties in the application of this method to rabbit liver extracts have been discussed, together with the factors which might possibly be concerned in catalysing the oxidation of GSH in such extracts. A comparison has been made between the values obtained for the GSH determined by manometric and iodate titration methods. It has been shown that the glyoxalase of rat and mouse liver is stable to prolonged oxygenation at 0° C. The toxicity of zinc and sulphosalicylic acid to the enzyme system has been investigated. The value of measurements of glyoxalase activities in rat and mouse liver extracts has been criticized. [ABSTRACT FROM AUTHOR]
Databáze: Complementary Index
Externí odkaz: