| Autor: |
Bajpai, Yashi, Kumar, Sandeep, Muthukumar, M., Rajan, S., Bajpai, Anju, Trivedi, Mala |
| Zdroj: |
Journal of Applied Horticulture; Jan-Apr2023, Vol. 25 Issue 1, p3-9, 7p |
| Abstrakt: |
Proteome-based characterization of vegetative and flower bud formation was utilized to identify and differentiate protein species with significant variable abundance during floral transition in mango cv. Dashehari using 2DE and corroborating the identified protein spots using gene expression analysis. Total soluble proteins were phenol-extracted from the vegetative and floral flush of mango cv. Dashehari and separated on 2D gels at pH 4-7. The protein spots with variable intensity were identified through SameSpots software. The protein sequences of differentially accumulated spots were identified based on PI and MW using Citrus sinensis proteome isoelectric focusing database. Furthermore, these protein sequences were used to conduct (tBLASTn) against Mangifera indica to predict the protein. Real time gene expression was done to corroborate identified proteins. Total 301 spots were detected, out of which 16 were identified as differentially expressed based on P value (P≤0.05) and a 2-fold change. These 16 protein spots were identified on the basis of in silico comparative mapping protein against genome of mango and citrus, a close relative. They were classified into eight categories viz., transcriptional regulation, phenylpropanoid pathway and cell wall /cytoskeleton metabolism-related proteins, hormone signalling, flowering time, signal-transduction, transport and protein synthesis associated to flowering. Five genes coding for shortlisted proteins were used for validation of results using gene expression analysis. SAM (S adenosyl methionine synthase) was found to up-regulated in floral flush, involved in the biosynthesis of polyamines has an association with flowering and stress responses. Furthermore, ARF (Auxin Response Factor) and serine/threonine kinase gene members were also found to play a critical role in determining the floral development process in mango, consistent with results obtained through 2DE. Protein species putatively involved in phenylpropanoid pathway were also identified, showing the process of mango flowering from a new perspective beyond the conventional view. This study of flowering-related proteomics provides an overview of the biological pathways and regulatory mechanisms associated with the developmental physiology of flowering. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Complementary Index |
| Externí odkaz: |
|
