| Abstrakt: |
Vascularization and the control of luteal and endometrial development are regulated by hypoxia‐inducible factors (HIFs) and vascular endothelial growth factor (VEGF) during pregnancy. In this study, the mRNA and protein expression levels of HIFs (HIF1A, HIF2A and HIF3A) and VEGF in goat uterine and ovarian tissues during various stages of pregnancy were evaluated. A total of 42 Hair goats were used and were allocated into six groups, namely embryo‐positive (G1), early pregnancy (G2), mid‐term pregnancy (G3), late pregnancy (G4), oocyte‐positive group (G5) and diestrus group (G6). The mRNA expression of the examined genes was evaluated by RT‐qPCR, and protein expression was evaluated by immunohistochemistry (IHC). In caruncles, HIF1A mRNA expression was greater in G1, G2 and G4 than in G3 (p <.05). HIF1A and HIF2A expression was greater in G1 than in G5 (p <.05). In cotyledons, HIF1A, HIF2A and HIF3A mRNA expression was greater in G2 and G3 compared to G4 (p <.05). In luteal tissue, HIF1A mRNA expression was greater in G1 and G2 than in G3 and G4 (p <.05). In the immunohistochemical examination, HIF1A, HIF2A, HIF3A and VEGF immunoreactions were detected in uterine and luteal tissues. Findings suggest that HIFs and VEGF are involved in the regulation of ovarian functions as well as the processes of implantation and placentation. [ABSTRACT FROM AUTHOR] |
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